In silico PCR Amplification

In silico PCR Amplification
Source code

Genome        Medicago sativa L. (CADL)
     Medicago truncatula v4
     Medicago truncatula v3.5.5
     Medicago truncatula v3.0
Input1       
Tile Size       
    the size of match that triggers an alignment
Step Size       
    spacing between tiles
Max Amplicon Size        bp
    Maximum size of PCR product
Minimum Perfect Match        bp
    Minimum size of perfect match at 3' end of primer
Minimum Good Match        bp
    Minimum size where there must be 2 matches for each mismatch